酶控制的单列dna成功通过蛋白质纳米洞-凯发k8官网

santa cruz, ca, usa and ¹, uk, 27 september 2010: research published this week in nature nanotechnology shows a new method of ²-controlled movement of a single ³ of ⁴ through a protein nanopore. the paper, by researchers at the university of california santa cruz (ucsc), represents a key step towards nanopore sequencing of dna ⁵（线，绳索） . the publication describes the observation of single ⁶ dna (ssdna) as it translocates through a protein nanopore, alpha hemolysin（溶血素） (ahl). movement of the ssdna was controlled by polymerase（聚合酶） -facilitated replication of individual dna ⁸. this movement could be ⁹ under electronic control. polymerase activity was shown to be blocked in solution when the ssdna was not at the nanopore opening, however capture of the strand by the pore removes a blocking strand of nucleotides（核苷酸） and allows the polymerase to function on the trapped strand.

ucsc researchers are ¹⁰ with oxford nanopore technologies ltd in the development of a new generation of electronic, single-⁷ dna sequencing technology. in the 'strand sequencing' method, current through a nanopore is measured as a dna polymer passes through that pore. changes in this current are used to identify the dna bases on the dna molecule, in sequence. this paper addresses a key challenge for dna strand sequencing: fine control of the translocation of the dna strand through the nanopore, at a rate that is consistent and slow enough to enable accurate identification of individual dna bases. the nature nanotechnology work shows for the first time that the motion of a strand can be controlled using electronic feedback（反馈） and that an enzyme can move a strand against a field while located on top of the nanopore.

"the techniques described in this paper are an advance towards electronic, single molecule dna sequencing of dna strands" said ¹¹ professor mark akeson of the university of california, santa cruz. "electronic control of dna translocation through a protein nanopore is a scientific goal that we have strived（努力，奋斗） towards for years and these methods are now forming the basis for further work in our laboratories. we are excited by our ¹² with oxford nanopore, whose parallel nanopore sensing strategy is impressive."